Sexual Assault Evidence Collection Protocol

 
 
 
 

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THE EVIDENTIARY (FORENSIC) AND MEDICAL EXAMINATION
A medical examination should be performed in all cases of sexual assault, regardless of the length of time that may elapse between the time of the assault and the examination. It is recommended that a forensic exam using the Sexual Assault Evidence Collection Kit be conducted if the assault occurred within 72 hours prior to the time of the examination. However, this determination should be made on a case-by-case basis through the consultation of medical personnel, law enforcement and crisis center advocate or support personnel.
If the sexual assault took place more than 72 hours prior to the examination, it is unlikely that trace evidence would still be present on the victim. However, evidence may still be gathered by documenting any findings obtained during the medical examination (such as bruises or lacerations), photographs and bite mark impressions (if appropriate), and statements about the assault made by the victim. Some victims may ignore symptoms that would ordinarily indicate serious physical trauma, such as internal injuries sustained by blunt trauma or foreign objects inserted into body orifices. Also, there may be areas of tenderness that will later develop into bruises.
Becoming infected with an STI or pregnant can be major concerns for sexual assault victims. Therefore, these issues must be addressed at the initial exam.
All supplies needed for a standard evidentiary examination are contained within the Sexual Assault Evidence Collection Kit. The Kit contains instructions. Follow the instructions to ensure a complete evidentiary examination. All potential forensic evidence should be collected. Supplies for additional evidence collection may be obtained from medical treatment facility stock or from a second Evidence Collection Kit. 
Attending Personnel
The attending medical personnel and, with the consent of the victim, a trained support person or advocate are the only persons who should be with the victim in the examining room. Although every effort should be made to limit the number of people in attendance during the examination, there may be instances when a victim requests the presence of a close friend or family member. If at all possible, these requests should be honored.
The least traumatic and least time consuming method of collecting physical evidence from sexual assault victims is to integrate the collection procedures into the medical examination. 
There is no medical or legal reason for a law enforcement representative, male or female, to observe the medical examination and evidence collection process. By virtue of the examination, the rectum and genitalia of the victim must be revealed for proper medical assessment and evidence collection. Subjecting victims to the observation of law enforcement personnel during these procedures, as well as having the law enforcement representative privy to the private communications between the victim and the examining medical personnel/support team, is unnecessary. Medical and law enforcement personnel communication should be reciprocal. Maintaining the chain of custody during the examination is the sole function of the attending medical personnel and requires no outside assistance.
The victim should be allowed to wash and dress before the in-depth investigative interview with the law enforcement officer.
Medical Report for Sexual Assault Examination
Throughout the evaluation and medical examination, the attending medical personnel should explain to the victim why questions are being asked, why certain medical and evidentiary tests may need to be performed and what treatment, if any, may be necessary.
 Vital signs and other initial information, such as the date and time of both the examination and the assault, should be recorded.
 A brief description of the medical details of the assault should be recorded. This description should include any oral, rectal, or vaginal penetration, whether the assailant penetrated the victim with finger(s) or foreign object(s), whether any oral contact occurred, and whether ejaculation occurred (if known).
 The victim’s account of what happened should be recorded accurately, briefly, and in the victim’s own words as much as possible.
 Information regarding the physical location of the assault should be recorded (car, rug, grass, pavement). This information will assist the attending medical personnel with an indication of where to look for evidence and what evidence to collect such as hair, fibers, and other trace material.
 Significant medical history of the victim should be recorded. Include any allergies, current medication, acute or chronic illness, surgery and any post-assault symptoms such as bleeding, pain, loss of consciousness, nausea, vomiting or diarrhea.
 Gynecological history information including menstrual history (last menstrual period, date and duration, menstrual cycle), pregnancy history (including evaluation of possible current pregnancy), and contraceptive history should be evaluated and recorded.
 If a victim is at risk of pregnancy, a urine pregnancy test should be done to establish a baseline for possible preexisting pregnancy.
 During the general physical examination, record all details of trauma, such as bruises, abrasions, lacerations, bite marks, blood or other secretions, with particular attention to the genital and rectal areas of both male and female victims.
 Common sites and types of injury include the breasts, the upper portion of the inner thighs, arms, wrists or legs, and injuries or soreness to the scalp area, back or buttocks as a result of being thrown against an object or onto the ground, and grab or retraining marks on the arms.
If penicillin is to be given as prophylaxis, it should not be delayed until the very end of the victim’s hospital examination. A victim may be allergic to penicillin but unaware of the allergy. It is recommended that this treatment be administered in time to allow for at least 30 minutes of victim observation.
Evidence Collection and Handling
If the assault has occurred within the recommended 72 hours or as determined on a case-by-case basis, use a Sexual Assault Evidence Collection Kit paying close attention to the following procedures. If a Kit is not available, the procedures described below should nevertheless be accomplished if considered appropriate by medical and law enforcement personnel.
Packaging and Refrigeration
In order to prevent the degradation of biological fluid stains and the loss of hair, fibers, or other trace evidence, clothing and other evidence specimens must be air dried and then sealed in paper or cardboard containers. Do not seal in plastic. If the containers are plastic, moisture remaining in the evidence items will be sealed in, making it possible for bacteria to quickly destroy biological evidence. Unlike plastic, paper “breathes” and allows moisture to escape. There are paper containers in the Kit. The paper and cardboard containers should be sealed with tape. Do not seal with staples as this will result in a biohazard to lab personnel. 
See Appendix A-1.
The Kit must be refrigerated (do not freeze) at all times after the evidence has been collected. The hospital or law enforcement must maintain this refrigeration until delivery to the crime laboratory. Standard transportation regulations should be followed to prevent freezing during winter months or heating during summer months.
Evidence Integrity (Sealing and Labeling)
The custody of the Kit and the specimens it contains must be accounted for from the moment of collection until the moment it is introduced in court as evidence. This is necessary in order to maintain the legally necessary “chain of evidence,” sometimes called “chain of custody,” or “chain of possession.” Its importance cannot be overemphasized because without being able to establish the “chain of custody,” evidence cannot be introduced in court.
It is also important that the names of those involved in the medical examination and collection and handling of evidence be legible on the evidence specimen and that enough information is recorded to locate them in the event their testimony is needed.
Proper handling and information recording are important. There must be a consideration for the chain of custody if the specimens collected are to be of later evidentiary value. “Chain of custody” need not be a concern if these simple practices are implemented:
 Properly label, preserve and store evidence specimens.
 Package, seal, and label evidence specimens, specifying the date, the time, the identity of the victim, the name of the attending medical personnel, and the area of the body from which specimen was taken.
 Minimize the number of persons handling the evidence specimens and have each person doing so initial the specimens.
 Note how and when samples are transferred from medical personnel to law enforcement and have law enforcement initial the external labels.
  Spermatozoa and Semen
In order to understand the importance of both spermatozoa and semen and the role they can play in the forensic analysis of sexual assault evidence, the following brief explanation is offered for review:
Semen is composed of cells and fluid, known as spermatozoa and seminal plasma, respectively.
The finding of spermatozoa is useful for two reasons:
 When present, it is positive indication that ejaculation occurred and that semen is present.
 When spermatozoa are motile (alive), it can be an indicator of the length of time since ejaculation.
The survival time of spermatozoa in the vaginal, oral and rectal orifices following ejaculation varies considerably in scientific studies. However, there is fairly wide consensus that they may survive for up to 72 hours or longer in the vagina (persisting longer in the cervical mucosa), and up to several hours or more in the rectal cavity, particularly if the victim has not defecated since the assault.
Seminal plasma is also useful for two purposes:
 In the absence of spermatozoa, seminal plasma components (p30 and acid phosphatase) can be used to identify semen. p30 is a prostatic antigen known to exist in the semen of humans and its presence is regarded as a conclusive indication of semen. Acid phosphatase is present in high levels in seminal samples but is considered only a presumptive test for the presence of semen because it also appears in samples that are not seminal in origin, such as vaginal fluid.
 Most of the genetic markers detected in semen and which are used to identify the possible donor, are located in seminal plasma.
Inasmuch as seminal plasma is produced in the ejaculates of all males, vasectomized or not, the forensic examiner is especially interested in the presence of seminal plasma. It is primarily the seminal plasma, not the spermatozoa, that gives evidence of the ABO blood type of secretors and the genetic markers of the donor of the specimen.
There has been a dramatic increase in the number of vasectomies. Many sexual assault offenders are sexually dysfunctional and do not ejaculate during the assault. Additionally, offenders may use a condom, have a low sperm count (frequent with heavy drug or alcohol use), ejaculate somewhere other than in an orifice or on the victim’s clothes or body, or fail to ejaculate if the assault is interrupted. Therefore, a lack of spermatozoa is not conclusive evidence that no assault occurred. It only means that spermatozoa may have been destroyed after being deposited or that it may never have been deposited at all.
Furthermore, the absence of semen means only that either no ejaculation occurred, for the reasons described above, or that various other factors contributed to the absence of detectable amounts of semen in the specimen. For example, there could have been a significant time delay between the assault and the collection of specimen, penetration of the victim could have been made by an object other than the penis, the victim could have inadvertently cleaned or washed away the semen, or the specimen could have been collected improperly.
Although the finding of semen, with or without the presence of spermatozoa, may corroborate the fact that a sexual act did take place and make a stronger case for the prosecution, its presence is not an absolute necessity for the successful prosecution of a sexual assault case.
NOTE: When collecting the vaginal specimen, it is important not to aspirate the vaginal orifice or to dilute the secretions in any way. Do not use lubricants or speculum aperatici. 
Presence of Sperm and Sperm Motility – Wet Mount and Permanent Smears
Wet mount slides are generally made immediately after specimens are collected, by combining a drop of the specimen from a swab with a drop of nutrient medium on the slide. The appropriate medical professional then examines the slide immediately to determine whether sperm is present, and if so, whether motile, non-motile or both. Motile sperm indicates a very recent ejaculation. It may be present for as short a time as 30 minutes and seldom beyond 6 to 8 hours. Several factors affect motility and/or presence of sperm. These factors include body temperature, amount of ejaculation, vaginal pH, presence of contraceptives, post-assault hygiene and whether the offender has had a vasectomy.
Permanent smears (dry mount slides) should be made from the samples obtained. These can be stained to help visualize sperm, kept indefinitely and examined by both medical and forensic personnel. Non-motile sperm may be seen on these slides where no sperm were seen on the wet mount. Non﷓motile sperm may be seen in vaginal or rectal secretions up to 12 to 20 hours following ejaculation – rarely might be found 48 to 72 hours later.
Wet mounts do not need to be retained. Permanent smears must be labeled and retained as evidence and placed in the Kit before sealing.
Analysis of Specimen – Specimen Collection
Unlike the clinical laboratories in most hospitals, forensic laboratories generally have more sophisticated equipment, specialized training and ultra-sensitive techniques that enable them to detect even minute traces of semen and spermatozoa. They are also able to conduct a genetic marker analysis of semen that the majority of hospital technicians are not equipped to perform. This information is entered into the CODIS with the Federal Bureau of Investigation. CODIS (Combined DNA Index System) is an electronic database of DNA profiles that can identify suspects.
Certain tests must be run immediately after evidence collection at the medical facility. The results of these hospital-run tests should be shared with forensic personnel.
It is not uncommon for the tests run at the forensic laboratory to contradict those run at the medical facility. For instance, because of the enhanced detection ability of the forensic laboratory traces of semen may be found despite negative test results from the hospital. Forensic personnel must then testify in court to explain the contradiction.
To minimize the chance of contradictions occurring, all medical and forensic specimens collected during the sexual assault examination must be kept separate both in terms of collection and processing. Those required only for medical purposes should be kept and processed at the Examining Hospital, and those required strictly for forensic analysis should be transferred with the Sexual Assault Evidence Collection Kit to the Forensic Laboratory for interpretation.
Use of Wood’s Lamp
A Wood’s Lamp is a long wave ultraviolet light that can be used to scan clothing or the body for evidence of dried or moist secretions, stains or injury. Semen usually exhibits a green or blue fluorescence under this light but may not when fresh. Clothing made of fluorescent synthetic material or washed in light optical density detergent may show seminal stained areas as absent or diminished fluorescence. If observations such as these have been made, diagrams should be used to record the location and extent of stains and/or injury.
If secretions are found on the hair, the matted hair can be cut and placed in a paper bindle. But be sure to relate to the victim what you are about to do and get consent from the victim before cutting hair. Thinner stains can be collected with the use of swabs moistened in distilled water that are then air dried and packaged in an envelope or tube. Secretions that are still moist can be collected with dry swabs to avoid dilution, air dried and packaged in the same way.
Use of Colposcope/Medscope
Genital trauma is a good indication of recent forced sexual contact. To obtain photographic evidence of injuries, the use of a colposcope is necessary. Recent research indicates that injury to the posterior forichette, the point of greatest stress in forced penetration, is characterized by an acute mounting injury. This injury is indicative of sexual assault. Other trauma sites may include labia minora, hymen, or fossa naviularis.
The colposcope can have video or camera equipment easily attached and these test results can be used as forensic documentation. The non-invasive nature of this procedure makes it valuable for use with all victims, especially children and the elderly.
Pregnancy Concerns and Sexually Transmitted Infections
A major fear for sexual assault victims is becoming pregnant. The attending medical personnel should discuss this with the victim and explain her options for pregnancy prevention. Most medical treatment facilities offer pregnancy prevention or the “morning-after” pill. This is for women who are at risk to becoming pregnant within the first 72 hours after the assault and who have had a negative pregnancy test. This option should be offered to the victim along with information regarding the risk of pregnancy and effectiveness and side effects of treatment.
During the medical examination, attending medical personnel should be mindful of the possibility that the sexual assault may lead the victim to contract an STI. 
Most clinicians recommend prophylactic treatment of STI’s rather than performing cultures for the following reasons: performing cultures at the initial exam has not proven helpful to the prosecution of the perpetrator; positive cultures at the initial exam have been used against the victim in court to indicate sexual promiscuity; STI cultures are expensive and time consuming; and victims often do not return for follow-up testing and treatment. 
Court-ordered Testing for Sexually Transmitted Infections
A court may order testing of any defendant charged with a sex offense (and any juvenile offender who is alleged to have committed certain crimes) to determine whether the defendant or alleged juvenile offender has any sexually transmitted disease. See Appendix I.
The court may order this testing only if the court receives a petition from the victim or from the prosecuting attorney with a written request from the victim. The suggested petition for use in North Dakota is attached as Appendix H.
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